An Attempt to Restore the Fertility of Miscanthus × giganteus

Miscanthus × giganteus is a popular industrial plant with great potential in ecological agriculture. It forms numerous rhizomes that are important the sequestration of carbon dioxide. The can be source lignin and cellulose, biomass for renewable energy production, used small garden architecture, or to strengthen banks landslides. Breeding this species difficult, as it sterile allotriploid 57 chromosomes. aim study was obtain fertile plants by treating its callus regenerants chromosome doubling agents such colchicine, oryzalin, trifluralin, caffeine at variable concentrations durations. Callus cells naturally showed large variations number chromosomes but only euploid regenerated plants. Treatment 1252 µM colchicine 18 h allowed obtaining two hexaploid shoots; however, they died before flowering. Colchicine oryzalin stimulated formation mixoploid shoots. investigated substances, except caffeine, were highly toxic M. 114 may die because high unfavorable species.